| 1 | The Baby From Krypton Intro (from Superman Radio Show [1940]) | Bud Collyer | 1:14 |
| 2 | What Are You Going to Do When You Are Not Saving the World? (from Man of Steel [2013]) | Hans Zimmer | 5:24 |
| 3 | Superman Theme (from Ruby-Spears Superman [1988]) | John Williams | 1:02 |
| 4 | Superman the Animated Series Theme (1996) | Shirley Walker | 1:03 |
| 5 | The Flying Sequence / Can You Read My Mind? (1978) | John Williams & Margot Kidder | 8:06 |
| 6 | Fight Night (from Batman V Superman: Dawn of Justice [2016]) | Hans Zimmer & Junkie XL | 4:20 |
| 7 | Batman Theme (1966) | Neal Hefti | 0:46 |
| 8 | Batman the Animated Series (1996) | Danny Elfman | 1:03 |
| 9 | The Ballad of Batman (from Batman: The Brave and the Bold [2002]) | John DiMaggio, Michael McCuistion, Lolita Ritmanis & Kristopher Carter | 2:17 |
| 10 | Batman and Robin: Batman Takes Over (intro [1949]) | Mischa Bakaleinikoff | 2:11 |
| 11 | Why Do We Fall? (from Dark Knight Rises [2012]) | Hans Zimmer | 2:04 |
| 12 | Christmas With the Joker / Jingle Bells Batman Smells (1992) | Shirley Walker & Mark Hamill | 0:35 |
| 13 | Joker Is Wild (1966) | The Sensational Guitars of Dan & Dale, Sun Ra & The Blues Project | 1:56 |
| 14 | Mischief (Harley Quinn’s Theme) [from Infinite Crisis Videogame [2015]] | Matthew Harwood | 0:51 |
| 15 | Lex Luthor’s Lair (1978) | John Williams | 2:33 |
| 16 | Penguin Makes Moves (From Gotham [2015]) | Graeme Revell & David E. Russo | 0:47 |
| 17 | Penguin’s Umbrella (1966) | The Sensational Guitars of Dan & Dale, Sun Ra & The Blues Project | 3:02 |
| 18 | Challenge of the Super Friends (1978) | Hoyt Curtin | 1:02 |
| 19 | The Theme of the Justice League of America (1966) | Arthur Korb | 3:11 |
| 20 | Supergirl Theme (2015) | Blake Neely | 1:11 |
| 21 | Robin’s Theme (1966) | The Sensational Guitars of Dan & Dale, Sun Ra & The Blues Project | 3:03 |
| 22 | The Adventures of Superpup (1958) | TV Pilot | 0:37 |
| 23 | DC’s Legends of Tomorrow Theme (2016) | Blake Neely | 0:50 |
| 24 | Metamorpho (1966) | Arthur Korb | 2:13 |
| 25 | Get Your Cape On (2015) | Jordyn Kane | 3:34 |
| 26 | The Flash TV Series Theme (1990) | Danny Elfman | 1:33 |
| 27 | The Flash Vs Arrow (2014) | Blake Neely | 5:07 |
| 28 | Wonder Woman (1996) | Arthur Korb | 1:25 |
| 29 | Wonder Woman TV Series Season 3 Theme (1978) | Charles Fox & Norman Gimble | 1:05 |