| 1 | Opening (Destroyed Science Academy Research Station) | Minako Hamano | 1:41 |
| 2 | The Last Metroid is in Captivity... | Dan Owsen | 0:10 |
| 3 | Theme of Super Metroid | Kenji Yamamoto | 3:24 |
| 4 | Arrival at the Space Colony | Kenji Yamamoto | 0:15 |
| 5 | Deserted Ceres Space Colony | Kenji Yamamoto & Minako Hamano | 0:29 |
| 6 | Raid on Ceres (Ridley ~ Escape) | Minako Hamano | 1:16 |
| 7 | The Space Colony Explodes ~ To Planet Zebes | Kenji Yamamoto | 0:31 |
| 8 | Planet Zebes - Arrival in Crateria | Kenji Yamamoto | 2:41 |
| 9 | Crateria - The Space Pirates Appear | Kenji Yamamoto | 3:38 |
| 10 | Brinstar - Overgrown with Vegetation Area | Kenji Yamamoto | 3:41 |
| 11 | Small Boss Confrontation BGM (Spore Spawn, Botwoon) | Minako Hamano | 2:14 |
| 12 | Item Room | Kenji Yamamoto & Minako Hamano | 0:53 |
| 13 | Brinstar - Red Soil Swampy Area | Kenji Yamamoto | 4:06 |
| 14 | Tension before a Confrontation | Kenji Yamamoto & Minako Hamano | 1:12 |
| 15 | Big Boss Confrontation BGM (Kraid, Crocomire, Phantoon) | Minako Hamano | 1:04 |
| 16 | Norfair - Burning Hot Lava Area | Kenji Yamamoto | 2:20 |
| 17 | Theme of Samus Aran, Space Warrior | Kenji Yamamoto | 4:08 |
| 18 | Wrecked Ship | Minako Hamano | 4:40 |
| 19 | Maridia - Rocky Underground Water Area | Minako Hamano | 2:56 |
| 20 | Maridia - Quicksand Underground Water Area | Minako Hamano | 3:58 |
| 21 | Norfair - Ancient Ruins Area | Kenji Yamamoto | 4:46 |
| 24 | Continue | Kenji Yamamoto & Minako Hamano | 0:23 |
| 25 | Mysterious Statue Chamber | Kenji Yamamoto | 1:38 |
| 26 | Tourian | Minako Hamano | 3:49 |
| 27 | Mother Brain | Minako Hamano | 2:20 |
| 29 | Planet Zebes Explodes | Kenji Yamamoto | 0:25 |
| 31 | Samus Aran's Appearance Fanfare | Kenji Yamamoto & Minako Hamano | 0:07 |
| 32 | Item Acquisition Fanfare | Kenji Yamamoto & Minako Hamano | 0:07 |
| 33 | Samus Aran's Final Cry | Kenji Yamamoto | 0:05 |
| 23 | Big Boss Confrontation BGM (Ridley, Draygon) | Minako Hamano | 1:14 |
| 22 | Chozo Statue Awakens | Kenji Yamamoto & Minako Hamano | 1:13 |
| 28 | Escape | Minako Hamano | 1:10 |
| 30 | Ending | Minako Hamano | 3:25 |
| X01 | Crateria Rainstorm | Kenji Yamamoto | 1:38 |
| X02 | Crateria Underground | Kenji Yamamoto | 2:41 |
| X03 | Wrecked Ship (Activated) | Minako Hamano | 4:42 |
| X04 | The Space Colony Explodes (Game Over) | Kenji Yamamoto | 0:09 |
| X05 | Etecoons Wall Jump Jingle | Kenji Yamamoto & Minako Hamano | 0:05 |
| X06 | Unknown (Junk Audio) | Kenji Yamamoto & Minako Hamano | 0:03 |