| 1 | Mule Skinner Blues (Blue Yodel No. 8) | Bill Monroe | 3:05 |
| 2 | You Won't Be Satisfied That Way | Bill Monroe | 2:36 |
| 3 | Uncle Pen | Bill Monroe | 2:21 |
| 4 | Blue Moon of Kentucky | Bill Monroe | 3:18 |
| 5 | Ole Slew-Foot | Jim and Jesse | 2:43 |
| 6 | Sweet Little Miss Blue Eyes | Jim and Jesse | 2:16 |
| 7 | Please Be My Love | Jim and Jesse | 3:02 |
| 8 | I Wish You Knew | Jim and Jesse | 2:40 |
| 9 | Love, Please Come Home | James Monroe | 2:21 |
| 10 | Train '45' (Heading South) | James Monroe | 2:36 |
| 11 | Bonny | James Monroe | 2:05 |
| 12 | When My Blue Moon Turns to Gold Again | James Monroe | 2:20 |
| 13 | Hit Parade of Love | Jimmy Martin | 2:56 |
| 14 | Mary Ann | Jimmy Martin | 3:00 |
| 15 | Sunny Side of the Mountain | Jimmy Martin | 2:53 |
| 16 | Free Born Man | Jimmy Martin | 3:17 |
| 17 | Tennessee | Jimmy Martin | 2:43 |
| 18 | Roll in My Sweet Baby's Arms | Lester Flatt | 2:51 |
| 19 | Feudin' Banjos | Lester Flatt | 2:09 |
| 20 | The Ballad of Jed Clampett | Lester Flatt | 2:05 |
| 21 | Roll on Buddy, Roll on | Bill & James Monroe | 3:03 |
| 22 | I Wonder Where You Are Tonight | Bill Monroe & Jim and Jesse | 2:59 |
| 23 | Orange Blossom Special | Bill Monroe & Carl Jackson | 2:27 |
| 24 | Fiddler Roll Call / Down Yonder | Bill Monroe | 4:07 |
| 25 | Soldier's Joy | Bill Monroe | 2:32 |
| 26 | Grey Eagle | Bill Monroe | 2:33 |
| 27 | Swing Low, Sweet Chariot | Bill Monroe | 4:03 |