| 1 | Ave Maria... Virgo Serena, Motet for 4 Parts | Josquin des Prez | 6:49 |
| 2 | Requiem (Missa Pro Defunctis), for 3 to 4 Voices (Introit, Kyrie, Gradual, Offertory Only): Introit: Re | Johannes Ockeghem | 5:40 |
| 3 | Nuper Rosarum Flores, Isorhythmic Motet for 4 Voices | Guillaume Dufay | 6:47 |
| 4 | O Salutaris Hostia | Pierre de la Rue | 1:44 |
| 5 | Quam Pulchra Es, for 3 Voices | Leonel Power | 4:19 |
| 6 | Veni Sancte Spiritus, Isorhythmic Motet for 4 Voices | John Dunstable | 6:27 |
| 7 | Himno XIX Pange Lingua IV | Antonio de Cabezón | 3:10 |
| 8 | Alma Redemptoris, Plainchant (Old Hall Manuscript) | [anonymous] | 2:07 |
| 9 | Salve Porta Paradisi for 3 Voices | Thomas Damett | 1:46 |
| 10 | In Monte Oliveti, Motet for 6 Voices, M. VI (S. Xi/187) | Orlande de Lassus | 4:35 |
| 11 | Tota Pulchra Es, Motet for 5 Voices (From Motets Book IV From Canticis Canticorum) | Giovanni Pierluigi da Palestrina | 2:15 |
| 12 | Vulnerasti Cor Meum, Motet for 5 Voices (From Motets Book IV From Canticis Canticorum) | Giovanni Pierluigi da Palestrina | 2:53 |
| 13 | De Tous Biens Plaine, Chanson | Hayne van Ghizeghem | 3:12 |
| 14 | Mille Regretz, Song for 4 Parts | Josquin des Prez | 2:05 |
| 15 | Il Bianco E Dolce Cigno, Madrigal for 4 Voices, S. 2/18 | Jacques Arcadelt | 2:03 |
| 16 | Nymphes Des Bois/Requiem Aeternam/Deploration De La Mort De Jehan Ockeghem, Lament for 5 Parts | Josquin des Prez | 5:50 |
| 17 | Clamabat Autem Mulier | Pedro de Escobar | 4:14 |