| 1 | Cançión (untitled) (à 6) | Francisco Guerrero | 2:44 |
| 2 | Cançión "Ecce sacerdos magnus" (à 5) | Philippe Rogier | 2:07 |
| 3 | Cançión (untitled) (à 6) | Cristóbal de Morales | 1:49 |
| 4 | Tiento del quinto tono (extract) | Cristóbal de Morales | 1:15 |
| 5 | Introitus - In medio ecclesiae | [anonymous] | 3:12 |
| 6 | Missa "Mille regretz": Kyrie | Cristóbal de Morales | 5:30 |
| 7 | Missa "Mille regretz": Gloria | Cristóbal de Morales | 4:23 |
| 8 | Oratio - Dominus vobiscum ... Deus, qui populo tuo | [anonymous] | 1:09 |
| 9 | Tiento del quarto tono (extract) | Antonio de Cabezón | 1:29 |
| 10 | Epistola: Lectio Epistolae beati Pauli Apostoli ad Timotheum | Antonio de Cabezón | 1:55 |
| 11 | Graduale - Cançión "Subiendo amor" | Francisco Guerrero | 1:08 |
| 12 | Cançión "Todos aman" | Francisco Guerrero | 1:15 |
| 13 | Evangelium - Dominus vobiscum ... Sequentia sancti Evangelii secundum Matthaeum | [anonymous] | 2:00 |
| 14 | Missa "Mille regretz": Credo | Cristóbal de Morales | 8:59 |
| 15 | Offertorium - Tiento del octavo tono | Antonio de Cabezón | 1:04 |
| 16 | O Doctor optime | Francisco Guerrero | 3:40 |
| 17 | Prefatio - Per omnia saecula saeculorum... Vere dignum et iustum est... | [anonymous] | 3:10 |
| 18 | Sanctus - Missa "Mille regretz": Sanctus | Cristóbal de Morales | 5:29 |
| 19 | Elevatio - "Mille regretz" | Nicolas Gombert | 2:48 |
| 20 | Pater noster - Per omnia saecula saeculorum... Pater noster... | [anonymous] | 2:14 |
| 21 | Missa "Mille regretz": Agnus Dei | Cristóbal de Morales | 6:58 |
| 22 | Communio - Fidelis servus et prudens | [anonymous] | 1:07 |
| 23 | Postcommunio - Dominus vobiscum... Ut nobis, Domine | [anonymous] | 1:06 |
| 24 | Ite, missa est - Ite, missa est... Deo gratias | [anonymous] | 0:51 |
| 25 | Recessional - Cançión (untitled) (à 6) | Philippe Rogier | 1:50 |
| 26 | Del modo de tañer a corcheas | Tomás de Santa María | 0:51 |
| 27 | Commemorative motet - Emendemus in melius | Cristóbal de Morales | 6:10 |