| 1 | The Pearls | Jelly Roll Morton | 3:26 |
| 2 | Roosevelt in Trinidad | The Atilla | 2:37 |
| 3 | Big Bad Bill | Emmett Miller | 3:09 |
| 4 | Any Old Time | Jimmie Rodgers | 2:48 |
| 5 | Diddy Wah Diddy | Blind Blake | 2:59 |
| 6 | Viola Lee Blues (Part 1) | Noah Lewis | 3:09 |
| 7 | The Girl I Love, She Got Long Curly Hair (Goin' to Brownsville) | Sleepy John Estes | 2:58 |
| 8 | The Bourgeois Blues | Lead Belly | 3:24 |
| 9 | How Can a Poor Man Stand Such Times and Live? | Blind Alfred Reed | 3:15 |
| 10 | Railroad Boomer | Riley Puckett | 2:47 |
| 11 | Vigilante Man | Woody Guthrie | 3:28 |
| 12 | Dark Was the Night, Cold Was the Ground | Blind Willie Johnson | 3:22 |
| 13 | You Can't Stop a Tattler, Part 2 | Washington Phillips | 2:48 |
| 14 | Face to Face That I Shall Know Him | Joseph Spence | 4:26 |
| 15 | Jesus on the Mainline | Mississippi Fred McDowell | 2:14 |
| 16 | Smack Dab in the Middle | The Jacks | 3:01 |
| 17 | Crazy 'bout an Automobile | Billy “The Kid” Emerson | 2:43 |
| 18 | Get Rhythm | Johnny Cash | 2:15 |
| 19 | Little Sister | Elvis Presley | 2:33 |
| 20 | Thirteen Question Method | Chuck Berry | 2:15 |
| 21 | It's All Over Now | Bobby Womack & The Valentinos | 2:47 |
| 22 | Down in the Boondocks | Billy Joe Royal | 2:35 |
| 23 | Mexican Divorce | The Drifters | 2:35 |
| 24 | The Girls From Texas | Jimmy Lewis | 2:41 |
| 25 | Poquito Fe | Flaco Jiménez & Toby Torres | 2:47 |