| 1 | Premonition of Fell Purpose [Opening (Destroyed Science Academy Research Station)] | Protricity | 3:30 |
| 2 | So It Begins… (Theme of Super Metroid) | Vig | 2:41 |
| 3 | Hostile Abduction [Big Boss Confrontation BGM (Ridley, Draygon)] | Suzumebachi & Protricity | 3:50 |
| 4 | Putting Down on Zebes (Planet Zebes - Crateria Arrival) | Protricity | 3:07 |
| 5 | Evil Aroused (Crateria - Space Pirates Appear) | Protricity | 4:17 |
| 6 | Full of Life (Brinstar - Plant Overgrowth Area) | Protricity | 3:37 |
| 7 | Unsettling Nature [Small Boss Confrontation BGM (Spore-Spawn, Botsoon)] | Jason Covenant | 3:22 |
| 8 | Traversing the Beyond (Space Warrior - Samus Aran’s Theme) | Danny Baranowsky & Protricity | 3:33 |
| 9 | Vast Inner Depths of Brinstar (Brinstar - Red Soil Wetland Area) | Protricity | 6:30 |
| 10 | Sudden Death [Big Boss Confrontation BGM (Kraid, Crocomire, Phantoon)] | Protricity | 1:54 |
| 11 | A Change and a Passing (Mysterious Statue Chamber / Tourian) | Protricity | 2:34 |
| 12 | Metal Fatigue (Wrecked Ship) | Children of the Monkey Machine | 4:17 |
| 13 | Noumenon (Maridia - Rocky Underground Water Area) | Avien | 2:49 |
| 14 | Ebb Tide Manifest (Maridia - Quicksand Underground Water Area) | Protricity | 3:56 |
| 15 | Pyroclastica (Norfair - Scorching Lava Area) | Children of the Monkey Machine | 2:28 |
| 16 | Braving the Flames (Norfair - Ancient Ruins Area) | ella guro | 4:57 |
| 17 | Perceived by Cold Intelligence (Mother Brain) | Protricity & zyko | 6:26 |
| 18 | Escaping Retribution (Escape) | Protricity | 4:01 |
| 19 | The Galaxy Is at Peace… (Ending / Samus Aran Appearance Fanfare) | Danny Baranowsky | 4:00 |